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Malachite Green Assay Kit
Cat No:K-1500 EAKIT | Brand:Echelon Biosciences | Pack Size:EA KIT
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Description
| Note | This product is for research use only. |
|---|---|
| Description | Echelon’s Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2, 000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples, standards, and controls. <span style="text-decoration: underline;">The kit is offered in three sizes:</span> <ol> <li>K-1500: includes 2 X 96-well plates, 1 vial of phosphate standard and a bottle of malachite green solution.</li> <li>K-1520: includes 1 X K-1500 + your choice of 2 X 100 ug lipid substrates.</li> <li>K-1530: includes 1 X K-1500 + your choice of 3 X 100 ug lipid substrates.</li> </ol> Suggested available substrates: <a href="https://echelon-inc.com/product/phosphatidylinositol-3-phosphate-dic8-pi3p-dic8/">diC8 PI(3)P</a>, <a href="https://echelon-inc.com/product/pi4p-dic8/">PI(4)P</a>, <a href="https://echelon-inc.com/product/pi5p-dic8/">PI(5)P</a>, <a href="https://echelon-inc.com/product/pi34p2-dic8/">PI(3, 4)P2</a>, <a href="https://echelon-inc.com/product/pi35p2-dic8/">PI(3, 5)P2</a>, <a href="https://echelon-inc.com/product/pi45p2-dic8/">PI(4, 5)P2</a>, <a href="https://echelon-inc.com/product/pi345p3-dic8/">PI(3, 4, 5)P3</a> The <a href="https://www.echelon-inc.com/product/malachite-green-solution/">Malachite Green Solution</a> can also be purchased separately. <table border="1" width="422" cellspacing="0" cellpadding="0"> <tbody> <tr> <td><b>Phosphatase Substrate </b></td> <td><b>Lipid Phosphatase Enzyme</b></td> </tr> <tr> <td>PtdIns(3)P</td> <td>Myotubularin, MTMR2</td> </tr> <tr> <td>PtdIns(4)P</td> <td></td> </tr> <tr> <td>PtdIns(5)P</td> <td></td> </tr> <tr> <td>PtdIns(3, 4)P<sub>2</sub></td> <td>PTEN</td> </tr> <tr> <td>PtdIns(3, 5)P<sub>2</sub></td> <td>OCRL1, 5Ptase IV, Synaptojanin</td> </tr> <tr> <td>PtdIns(4, 5)P<sub>2</sub></td> <td>MTMR2, PTPRQ</td> </tr> <tr> <td>PtdIns(3, 4, 5)P<sub>3</sub></td> <td>PTEN, SHIP1, SHIP2, SKIP, PTPRQ, 5ptase II</td> </tr> </tbody> </table> <object id="wobj-318-k-1500" style="width: 100%; height: 96px; background-color: [rgb color];" data="https://www.bioz.com/v_widget_2_5/318/k-1500" type="text/html" width="300" height="150"></object> <div id="bioz-w-pb-k-1500-div" style="width: 100%; display: none;"><a id="bioz-w-pb-k-1500" style="font-size: 12px; text-decoration: none; color: transparent;" href="https://www.bioz.com/" target="_blank" rel="noopener noreferrer"><img style="width: 11px; height: 11px; vertical-align: baseline; padding-bottom: 0px; margin-left: 0px; margin-bottom: 0px; float: none; display: none;" src="https://cdn.bioz.com/assets/favicon.png" /> Powered by Bioz</a><a style="font-size: 12px; text-decoration: none; float: right; color: transparent;" href="https://www.bioz.com/result/k-1500/product/Echelon Biosciences/?cn=k-1500" target="_blank" rel="noopener noreferrer"> See more details on Bioz</a></div> |
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