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MeOSuc-Ala-Ala-Pro-Val-4MbNA (Pancreatic Elastase Substrate, fluorogenic)
Cat No:881-19 5MG | Brand:Echelon Biosciences
Description
| Pack Size | 5 mg |
|---|---|
| Storage Temperature | -20 degree C or below |
| Note | This product is for research use only. |
| Description | Molecular Weight: 625.31 Salt Form: N/A Purity: >96% Sequence (3-letter): MeOSuc-Ala-Ala-Pro-Val-4MbNA Sequence (1-letter): MeOSuc-AAPV-4MbNA Storage: -20 °C or below Pancreatic Leukocyte Elastase Substrate (MeOSuc-AAPV-4MbNA), aka Elastase Substrate 1, is a highly sensitive fluorogenic substrate for pancreatic leukocyte elastase (neutrophil elastase). Pancreatic leukocyte elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy bacteria as well as host tissue. MeOSuc-AAPV-4MbNA can be used for in vitro assays with purified enzyme or with biological fluids or conditioned medium. This product is not a substrate for cathepsin G or chymotrypsin. Activity is quantified by release of free fluorescent 4-Methoxy-β-naphthylamine (4MβNA) which excites at 335-350 nm and emits at 410-440 nm. <h4>References</h4> 1.<a href="http://www.ncbi.nlm.nih.gov/pubmed/11827996Adkison, > A.M. et al. (2002) “Dipeptidyl peptidase I activates neutrophil-derived serine proteases and regulates the development of acute experimental arthritis” J. Clin. Invest. 109 (3): 363–371.</a> 2.<a href="http://www.ncbi.nlm.nih.gov/pubmed/23231964"> Lonky, S. A and Whole, H. (1983) “Regulation of elastolysis of insoluble elastin by human leukocyte elastase: stimulation by lysine rich lugans, anionic detergents and ionic strength” Biochemistry 22: 3714.</a> 3. <a href="https://www.researchgate.net/publication/231451426_Catalysis_by_human_leukocyte_elastase_Rate-limiting_deacylation_for_specific_p-nitroanilides_and_amides">Stein, R. L., Viscarello, B. R., and Vildonger, R. A. (1984) “Catalysis by human leukocyte elastase. II. Rate limiting deacylation for specific p-nitroanilides and amides” J. Am. Chem. Soc.. 106: 796.</a> |
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