BTL Biotechno Labs Pvt. Ltd.- Your reliable Source of Biotin Assay Medium
Biotin Assay Medium is a specialised biotin-free dehydrated designed culture medium rich with all essential nutrients required for the growth of Lactobacillus plantarum ATCC 8014. As it lacks biotin, it is specifically formulated for the microbiological quantification of biotin concentration in pharmaceuticals, food and feed.
Key Principles Involved:
Lactobacillus plantarum requires biotin (Vitamin B7) for growth.
- When biotin is added at higher concentrations, the organism shows linear growth.
- Growth can be measured by:
- Turbidimetric method (optical density (OD) measurement)
- Titrimetric method (acid production measurement)
We compare the microbial growth response to a biotin standard curve, allowing quantification of biotin in unknown samples.
Physical and Chemical Characteristics
| Parameter | Description |
| Appearance | White to off-white, homogeneous free-flowing powder |
| Solubility | Clear, colorless to light amber solution (slight precipitate possible) |
| pH | As reported by manufacturer |
Preparation Methodology:
- Dissolve 7.5 g medium in 100 ml distilled water.
- Heat with stirring and boil for 2–3 minutes.
- Dispense 5 ml into tubes
- Add biotin standards or test samples
- Adjust the total volume to 10 ml and autoclave at 121°C for 5 minutes.
Storage
Prepared medium at 4°C, use shortly after preparation
Role of Biotin Assay Medium in Multivitamin Preparation
The medium plays a vital role in quality control and standardization of multivitamin formulations.
- Quantification of Biotin Content
Multivitamin products contain biotin in microgram quantities. Medium is used to:
- Verify brand claim accuracy
- Measure actual biotin concentration
- Detect under- or over-fortification
2. Quality Control Testing
Before release of multivitamin batches:
- Biotin levels are tested using microbiological assay
- Ensures compliance with pharmacopeial standards
- Confirms product potency and stability
3. Stability Studies
During shelf-life studies:
- Biotin degradation over time can be monitored
- Helps determine expiration date
4. Detection of Bioavailable Biotin
Unlike chemical assays, microbiological assays measure:
✔ Biologically active biotin
✔ Not just total chemical content
This is especially important in:
- Complex multivitamin syrups
- Fortified foods
- Nutraceutical capsules
Why Microbiological Assay is Preferred?
- High sensitivity (detects microgram levels)
- Measures biological activity
- Industry protocols in many pharmacopeias (USP, AOAC)
The Biotin Assay Medium has applications in academic, clinical, pharmaceutical, and industrial microbiology for accurately determining biotin concentration. It supports reliable vitamin analysis essential for research, quality control, and production processes.
Acknowledging its importance in both academic and industrial applications, BTL Biotechno Labs Pvt. Ltd. is the top distributor of Biotin Assay Medium in India. A leading supplier of life science research products, BTL Biotechno Labs Pvt Ltd. offers high-quality Biotin Assay Medium at competitive prices.
BTL Biotechno Labs Pvt. Ltd. maintains a standing inventory of Biotin Assay Medium, ensuring ready availability and immediate delivery to support research and production needs.
We are committed to supporting the scientific community in advancing the research across pharmacology, biochemistry and life sciences.
For product enquiry, please reach out to us at info@biotechnolabs.com
For product details, please connect with us at info@biotechnolabs.com.
