iLite® Cell-Based Solutions for the Quantification of Drug Activity & Immunogenicity by Svar Life Science

The iLite® cell-based solutions by Svar Life Science are based on the iLite technology, engineered cell-based assay system with a dual reporter gene readout. They offer the ease of use and robustness of a Ligand Binding Assay and can be developed for virtually any pharmaceutical target and allows an easy, rapid and accurate test format for measurement and quantification of drug activity and immunogenicity. BTL Biotechno Labs, as a trusted distributor, plays a pivotal role in bringing Svar Life Science products in India.

Cell-Based Assay & Reporter Gene Assays

Cell based assays are defined as any assay that takes place within a living cell. Because this definition is so general, there are thousands of different cell-based assays. Reporter-gene assays are a specific kind of cell-based assay with an engineered system that incorporates a regulatory element that control a given gene and that use an easily detectable readout like luciferase.

Assay Ready Cells - As easy as 1-2-3

The iLite® cell-based solutions are supplied as cryopreserved assay ready cells, that are harvested and frozen at the same time, which means a higher reproducibility and overall superior performance compared to cells in culture can be obtained

Thaw: The iLite® cell-based solutions are supplied as cryopreserved assay ready cells, that are harvested and frozen at the same time, which means a higher reproducibility and overall superior performance compared to cells in culture can be obtained

Plate: Next, plate your cells and add your sample. The cells contain a secondary reporter gene that can be used as a powerful tool for normalizing results and to compensate for differences in cell numbers. This means that you can be confident that you get a target-specific signal.

Incubate: Finally, incubate and read your assay. The combination of excellent target specificity and a high signal-to-noise ratio ensures a biologically relevant functional assay.

iLite® Cell-Based Portfolio

T-Cell Activation Bioassays

The iLite CD3 Effector Cell line is a reporter gene cell line that offers a convenient and powerful way of measuring the efficacy antibodies have to elicit T cell activation through CD3 interactions. The iLite T-Cell Activation Bioassays is a dual cell system based on bioluminescent cell-based assays.

The portfolio consists of cell lines provided in an “assay-ready” format for a rapid and convenient workflow and a further reduction in assay variability and can be used for the discovery and development of novel biologics such as bispecific antibodies and CAR-T cell therapies.

• Potency lot release and
• Antibody screening
• Assessing the comparability between innovators and biosimilars

Product List:

CD19 (+) [BM5025], CD19 (-) [BM5026], CD20 (+) [BM5010], CD20 (-) [BM5015] HER2 (+) [BM5011] , HER2 (-) [BM5016], EGFR (+) [BM5035], EGFR (-) [BM5036], mTNF-α (+) [BM5013], mTNF-α (-) [BM5014], mVEGF (+) [BM5017], mVEGF (-) [BM5018]

Fc Effector Activity Bioassays

The iLite Fc Effector Reporter Gene Bioassays Portfolio consists of a range of reporter gene cell lines and antigen-specific target cells that can be used in biologically relevant, MOA-reflective assays to measure the Fc effect of antibodies and other biologics that specifically bind and activates Fc receptors.

They are based on a Luciferase reporter gene system and offer a convenient and powerful way of measuring the efficacy of antibodies to elicit ADCC and ADCP in vitro.

The iLite® Fc Effector and Target cell lines Portfolio encompass Effector cells and positive and negative Target cells engineered to work optimally together - creating a system that is greater than the sum of its parts.

Effector Cells

ADCC Effector FcγRIIIa (V) [BM5001]
ADCP Effector FcγRIIa [BM5004]
CD3 Effector [BM5005]

Target Cells

ADCP Bioassays

The iLite ADCP product portfolio is based on a reporter gene system and offers a convenient and powerful way of measuring the efficacy antibodies have to elicit ADCP through the CD32/ FcyRIIa receptor in vitro.

The FcγRIIa ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcγRIIa. The product portfolio consists of cell lines provided in an “assay-ready” format for a rapid and convenient workflow and a further reduction in assay variability, enabling their use in antibody screening, characterization, stability, and potency studies, and potency studies. The FcγRIIa ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcγRIIa. The product portfolio consists of cell lines provided in an “assay-ready” format for a rapid and convenient workflow and a further reduction in assay variability, enabling their use in antibody screening, characterization, stability, and potency studies.

ADCC Bioassays

The iLite ADCC product portfolio is based on a reporter gene system and offers a convenient and powerful way of measuring the efficacy antibodies have to elicit ADCC through the CD16/ FcyRIIIa receptor in vitro. The iLite ADCC product portfolio is based on a reporter gene system and offers a convenient and powerful way of measuring the efficacy antibodies have to elicit ADCC through the CD16/ FcyRIIIa receptor in vitro.

Gene Therapy Solutions

Svar is in a unique position to offer assistance in any gene therapy project. They have robust platforms suited for all phases of drug development, as well as the experience and knowledge from our diagnostic and bioanalytical services. They are currently developing cell-based assays for immunogenicity and potency testing. Furthermore, they create custom projects tailored to your specific needs to create truly tailor-made assays. Their Bioanalytical Services offer flexible solutions adapted to your needs and requirements.

Gene therapy introduces a number of analytical challenges that are not encountered with conventional biopharmaceutical products. Complex and time-consuming bioassays are needed throughout the study to assess product potency, confirm product identity, identify impurities and test for immunogenicity. With the iLite Gene therapy solution they offer advanced analytical methods to address these challenges. BTL Biotechno Labs, serving as an esteemed distributor in India, plays a pivotal role in providing Svar Life Science distributor in India.

Services

Immunogenicity testing
Potency testing
Bioanalytical Services.

Custom Cell-based Services

Svar Life Science combines research and development, manufacturing, and regulatory affairs with quality control and quality assurance in a small, cross-functional organization.

They have over 15 years of experience working with cell lines. In addition to their off-the-shelf products, they have developed custom assays for a wide range of targets and customers. BTL Biotechno Labs, an esteemed Svar life science supplier in India, delivers cutting-edge solutions to advance scientific research and innovation.

We, BTL Biotechno Labs Pvt. Ltd., as a channel partner of Svar Life Science, provide their complete range of ready-to-use cell-based solutions for an easy, rapid, and accurate test for the measurement and quantification of drug activity and immunogenicity to the Indian scientific community.

For more details, follow the link below:

iLite® Cell-Based Solutions (svarlifescience.com)