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sgRNA

BTL Biotechno Labs Pvt. Ltd. provide one-stop services ranging from single sgRNA/sgRNA library design/synthesis and lentivirus packaging to bioinformatics analysis and much more.

Services

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We offer a wide range of services, including ready-to-use sgRNA, sgRNA plasmids, and sgRNA libraries

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We provide single-sequence and sgRNA library designs for gene knockout, repression, and activation in several species

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We provide customized design of sgRNA for a gene family or genome region.


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sgRNA design & synthesis for different species, including animals, plants, and microbes


Other Services:

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Human Genome Knockout Libraries

CRISPR technology is the third generation of gene editing technology after ZFN and TALEN. It is widely employed in several sectors, including the study of gene function, medication development, crop breeding, molecular diagnosis, and precision medicine. The use of CRISPR libraries for genome function screening has long been a well-liked research technique, as described by renowned journals worldwide.

CRISPR Library assesses and classifies more than 300,000 different sgRNAs and generates an optimized human minimal genome knockout library by combining the standardized resources of large-scale CRISPR screening and several efficiency indicators. There were 200 non-targeted sgRNAs and a total of 37,522 optimized sgRNAs for the 18,761 genes that were targeted.

Advantages:


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1. Accurate sgRNA Design

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2. Optimized Vector


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3. Cost-Effective

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4. One-Stop Service Solution

5. Rigorous Quality Control

Service Specifications

  • Sub library A: A total of 74,987 sgRNA sequences—18,913 targeted genes, 74,552 sgRNA sequences, and 435 control (non-targeted) sgRNA sequences.
  • Sub Library B: A total of 71,483 sgRNA sequences—18,334 targeted genes, 71,048 sgRNA sequences, and 435 control (non-targeted) sgRNA sequences.
  • Sub Library C: A total of 37,722 sgRNA sequences—18,761 targeted genes, 37,522 sgRNA sequences, and 200 control (non-targeted) sgRNA sequences.

Microbial Genome

The genomes of microorganisms contain thousands of genes. Finding the relationship between these genes and phenotypes using high-throughput and affordable technologies is important. CRISPR interference, or activation library, is a potent tool for the investigation of microbial cell factories that can examine the related gene groups of a certain phenotype under several screening conditions.

BTL Biotechno Labs Pvt. Ltd. offers efficient and accurate microbial genome editing services, including an E. coli genome CRISPR interference library, a yeast genome interference library, and a yeast genome activation library.

Standard Protocol:

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Service Specifications:

  • E. coli genome CRISPR interference library: 55,671 sgRNA sequences and 400 control (non-targeted) sgRNA sequences.
  • Yeast genome interference library: 61,094 sgRNA sequences
  • Yeast genome activation library: 61,094 sgRNA sequences

Microbial Editing

Microbial genome editing is a cutting-edge technology that has completely changed the world of biotechnology. We provide services for the development of microbial strains that produce high-value products like biofuels, drugs, and bioplastics by altering DNA sequences. We provide a cutting-edge genome editing service that guarantees precision down to the base pair level, leaving no selection marker behind and allowing you to edit any gene .

Service Specifications:

  • E.coli: Gene Knock-out & Knock-in
  • Yeast: Gene Knock-out & Knock-in
  • Guide RNA Design

SsDNA Synthesis

ssDNA (single-stranded DNA) is frequently used as a homologous recombination repair template in order to achieve site-directed repair and long fragment knock-in in gene editing.

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Advantages:

  • Sequence Accuracy
  • Non-Toxic Enzymatic Approach
  • Customized

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