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Gene Synthesis

Gene synthesis is the technique of artificially constructing a specific DNA sequence in a lab setting. We offer customised gene synthesis services to meet the needs of the scientific community in India. Our gene synthesis service is fast, precise, and reliable, making use of the most up-to-date tools and techniques to guarantee the precise construction of the desired DNA sequence. We provide several other services, such as codon optimisation, cloning, and gene assembly, to allow you to customise your gene synthesis project. We ensure that your synthesised genes are of the highest quality using cutting-edge tools and modern methods, allowing you to move forward confidently with your research goals.

BTL Biotechno Labs Pvt. Ltd. offers the knowledge and resources to suit your demands, whether you require a single gene or a large-scale gene synthesis project. Our gene synthesis services are reasonably priced and customised to meet your valuable time and financial requirements.

To find out more about how we can assist you with your gene synthesis needs, get in touch with us.

Features:

• We offer free subcloning into the majority of commercial vectors as part of our gene synthesis services. Additionally, we provide free vector deposit services that may be valuable for future projects and streamline your gene synthesis process.
• By choosing BTL Biotechno Labs Pvt. Ltd. for your gene synthesis needs, you can enjoy significant cost savings of up to 15–40% per gene compared to other providers.
• We have experience with a wide range of gene synthesis applications, including those in the biotechnology, agriculture, and medical industries.

Standard Gene Synthesis Process

Other Services:

Cloning

BTL Biotechno Labs Pvt. Ltd. offers a streamlined and efficient cloning process that enables the cloning of any desired genetic sequence into any site of any vector. Cloning services meet the needs of researchers across a variety of fields, providing you with the flexibility and customization you need for your specific research objectives.

Mutagenesis

Services include: insertion, deletion, arbitrary editing & site-directed mutagenesis and custom services.

Plasmid Preparation

We offer premium plasmid construction services that are tailored to your research project's particular requirements. We provide cutting-edge technologies and optimised workflows to make sure that your plasmids are prepared to the highest quality standards, whether you need them at a milligramme or gramme scale.

We provide services in Protein Expression and Purification

DNA Synthesis Platforms:

NG Codon Optimization

Codon optimization technology improves the protein expression level in most host cells

Next Generation Gene Synthesis

High throughput and cost-effective

Synthetic DNA Libraries

All types of mutant/variant libraries with diversity of up to 1014 confirmed by sequencing analysis.

Vector Construction

shRNA, TALEN, CRISPR-Cas9 construction and validation.

shRNA construction

• Cell-based shRNA services
• Lentivirus-based shRNA services
• Adenovirus-based shRNA services
• Stem cell-based shRNA services
• RNAi effects in stem cells

TALEN construction

• TALEN plasmid construction
• TALEN-based target locus function validation
• Construction of stable cell lines
• Stem cell construction

CRISPR-Cas9 construction

• CRISPR-Cas9-based sgRNA design.
• CRISPR-Cas9-based plasmid construction
• CRISPR-Cas9-based target locus validation
• CRISPR-Cas9-based stable cell line construction
• CRISPR-Cas9-based stem cell construction

Small Genome Synthesis & Assembly

A number of researchers discovered that DNA segment assembly in yeast has a lot of potential as a convenient approach. Without the use of DNA polymerases or ligases, yeast can connect several DNA segments automatically, allowing the construction of plasmids larger than 10 kb conceivable quickly. Therefore, the use of yeast homologous recombination technology is extremely advantageous for the quick and highly effective assembly of lengthy DNA fragments and genomes.

Molecular Biology Services

Plasmid DNA Preparation

• Fast delivery, starting at day 1
• Low endotoxin level
• Diversity of scale
• Premium quality

PCR Cloning & Subcloning

• 100% sequence accuracy
• Customizable service: no limits
• Cost-effective
• Professional technical support

Site-Directed Mutagenesis

• Vast region: 40bp region as 1 mutation
• Precise mutation: error-free
• Sequence up to 150Kb in length

Vector Construction

• Any vector: 100% accuracy
• shRNA construction
• TALEN construction
• CRISPR-Cas9 construction

RNA Synthesis

RNA has been extensively used in many biological research domains, including the study of gene function and the development of novel therapeutics. The demand for top-notch synthesis services has grown significantly as interest in RNA research has increased. Although the production of RNA and DNA is similar, RNA has a low coupling efficiency and is easily degraded.

We provide functional siRNA synthesis, miRNA synthesis, in vitro transcription synthesis, and RNA oligo synthesis with a range of modifications and delivery parameters. Every synthesised sequence goes through a standardised production process and is subject to stringent quality checks. We promise to provide the most economical price to meet your diversified needs without sacrificing overall quality.

In-Vitro Transcription

Intracellular mechanism of siRNA, shRNA and miRNA

Services Specification

Custom RNA Oligos: Synthesis of single/double stranded RNA as well as long RNA sequences.

siRNA/miRNA Synthesis: Premium quality siRNA/miRNA synthesis products, as well as corresponding NC products.

CRISPR-Cas9 sgRNA Synthesis: The synthesized sgRNA can be transformed into the cell along with Cas9 protein or Cas9 mRNA to perform the downstream targeting.

RNA Modification: Comprehensive RNA modification and labeling services, such as amino, biotin, FAM, HEX, BHQ,Cy3,Cy5, etc.