RbcL | Rubisco positive control/quantitation standard
SKU: BTL-AGR-A-00017 |
Brand: Agrisera
Product Description
| Cat Number | AS01 017S |
|---|---|
| Category | Primary Antibody |
| Pack Size | 100 µl |
| Description | Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.Source of Rubisco standard: Rubisco protein was purified directly from a plant tissue - spinach. |
| Applications | Western blot (WB) |
| Form | Lyophilized in glycerol. |
| Molecular Weight | 52.7 kDa |
| Dilution Range | Standard curve: three protein standard loads are recommended.For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains (standard concentration 7.9 µg/mL). Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized does not require heating before loading on the gel or addition of any buffer.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing. |
| Reconstitution | For reconstitution add 90 µl of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |
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