HighQC™ Human Mesenchymal Stem Cell-Expressing OCT3/4
SKU: BTL-AG-A-02923 |
Brand: Accegen
Product Description
| Cat Number | ABC-SC0032T |
|---|---|
| Category | Stem Cells |
| Pack Size | 1 vial |
| Product Description | It is well-established that induced pluripotent stem cells may be generated by co-transfection of four genes: Oct3/4, Sox2, Kfl4 and c-Myc, into somatic adult cells resulting in re-programming of these cells to pluripotency equivalent to that of embryonic stem cells. It has recently been shown by independent research including results from our laboratory that over-expression of a single gene, Oct3/4, in adult neural or mesenchymal stem cells also results in enhanced differentiation capacity including pluripotency. This development simplifies iPS generation and may reflect levels of endogenous expression of Sox2, Kfl4 and c-Myc in adult stem cells that are multipotent. Also, since the Oct3/4 promoter has well-known response elements and transcription control mechanisms, re-programming based on environmental cues is now feasible through use of small molecular agents that selectively activate expression of Oct3/4. Our human MSCs expressing Oct3/4 allow further investigation of iPSC mechanisms. Optimizing your hMSC Cultures: 1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2.. 2) Use of our MSCGroTM media is highly recommended. 3) Subtrate-We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent. |
| Specie Reactivity | Human |
| Note | The product is for research use only |
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