Product Description
| Cat Number | AP231-23-V4 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 6×500 units |
| Description | TransStart® FastPfu Fly DNA Polymerase is a hot start, ultra high-fidelity DNA Polymerase used for fast PCR. Compared withTransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity. 2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors. • Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. • PCR products can be directly cloned into pEASY ®-Blunt vectors. • Amplification of genomic DNA fragment up to 15 kb. • Amplification of plasmid DNA fragment up to 20 kb. Applications • Complex templates, GC/AT-rich templates. • Ultra high-fidelity and fast PCR, blunt end cloning, site-directed mutagenesis. • Long fragment amplification. Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Zhang A, Shan T, Sun Y, et al. Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors[J]. Plant Biotechnology Journal, 2023.(IF 13.80) 2 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022.(IF 63.714) 3 Wang D, Yan F, Wu P, et al. Global profiling of regulatory elements in the histone benzoylation pathway[J]. Nature Communications, 2022.(IF 14.919) 4 Niu L, Shen W, Shi Z, et al. Three-dimensional folding dynamics of the Xenopus tropicalis genome[J]. Nature Genetics, 2021.(IF 38.33) 5 Jin S, Fei H, Zhu Z, et al. Rationally designed APOBEC3B cytosine base editors with improved specificity[J]. Molecular cell, 2020.(IF 15.58) 6 Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13) |
| Note | The product is for research use only |
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