2×TransStart® FastPfu Fly PCR SuperMix(-dye)
SKU: BTL-TB-MB-00095 |
Brand: Transgen Biotek
Product Description
| Cat Number | AS231-01-V4 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | 1 ml |
| Description | This product contains TransStart® FastPfu Fly DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. It has the features of high amplification efficiency and fast amplification speed (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification). The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water for amplification. The amplified product of 2×TransStart®FastPfu Fly PCR SuperMix (-dye) can be cloned directly into pEASY®-Blunt series of vectors. The amplified product of 2×TransStart®FastPfu Fly PCR SuperMix (+dye) can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning. • Reduce PCR operation time. •Avoid contamination caused by multi-step operation. •TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. •Amplification of genomic DNA fragment up to 15 kb. •Amplification of plasmid DNA fragment up to 20 kb. Highlights Fast, ultra-high fidelity, high specificity, good stability. Applications • Ultra high fidelity PCR • Site-directed mutagenesis • Blunt end cloning • Complex templates • GC/AT-rich templates • Long fragment amplification Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| Note | The product is for research use only |
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