Non-biotinylated SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG)
SKU: BTL-KB-P-01100 |
Host: SARS-COV-2 |
Brand: Kactus Bio
Product Description
| Cat Number | COV-VM4SG |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G). |
| Target | Spike S1 |
| Host | SARS-COV-2 |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Molecular Mass | The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Gln14-Arg683(D614G) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | QHD43416.1 |
| Amino Acid Sequence | Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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