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α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit

SKU: BTL-EL-AK-02332 | Brand: Elabscience
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Product Description

Cat Number E-BC-K083-M_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Colorimetric method
Research Field Mitochondria Function;Tricarboxylic Acid(TCA)Cycle
Sensitivity 8.3 U/L
Detection Range 8.3-42.3 U/L
Sample Type serum;plasma;animal;plant tissue
Assay Length 40 min
Target Synonyms α-KGDH
Reader Microplate reader
Test Principle α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm
Storage This product can be stored at -20°C for 12 months with shading light.
Technical Manual View Document
Msds View Document
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Note The product is for research use only
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