Glutathione Peroxidase (GSH-Px) Activity Assay Kit
SKU: BTL-EL-AK-02342 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K096-M_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Colorimetric method |
| Research Field | Oxidative stress;Plant Physiology Series;Ferroptosis |
| Sensitivity | 34.34 U |
| Detection Range | 34.34-1036.64 U |
| Sample Type | serum;plasma;cells;cell culture supernatant;tissue |
| Assay Length | 40 min |
| Target Synonyms | GSH-PX |
| Reader | Microplate reader |
| Test Principle | Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH. |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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