Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
SKU: BTL-EL-AK-02347 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K097-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Oxidative stress;cuproptosis;Ferroptosis |
| Sensitivity | 0.36 μmol/L |
| Detection Range | 0.36-30 μmol/L |
| Sample Type | serum;plasma;animal tissue;red blood cells;cultured cells |
| Assay Length | 100 min |
| Target Synonyms | T-GSH;GSSG |
| Reader | Microplate reader |
| Test Principle | GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle. |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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