Product Description
| Cat Number | E-BC-K161-S_50Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 50Assays |
| Detection Method | Colorimetric method |
| Research Field | Glycolysis and Lipid Metabolism |
| Sensitivity | 0.32 μmol/mL |
| Detection Range | 0.32-70 μmol/mL |
| Sample Type | plant tissue |
| Assay Length | 40 min |
| Reader | Spectrophotometer |
| Test Principle | Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose. |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
Share Item: