Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
SKU: BTL-EL-AK-02537 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K236-M_500Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 500Assays |
| Detection Method | Colorimetric method |
| Research Field | Liver and Renal Function |
| Sensitivity | 1.10 IU/L |
| Detection Range | 1.10-72.30 IU/L |
| Sample Type | serum;plasma;animal tissue;cells;cell culture supernatant |
| Assay Length | 80 min |
| Target Synonyms | AST;GOT;Glutamic-oxalacetic transaminase |
| Reader | Microplate reader |
| Test Principle | AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| References | View Document |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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