Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
SKU: BTL-EL-AK-02539 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K236-S_100Assays |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 100Assays |
| Detection Method | Colorimetric method |
| Research Field | Liver and Renal Function |
| Sensitivity | 0.38 IU/L |
| Detection Range | 0.38-72.30 IU/L |
| Sample Type | serum;plasma;animal tissue |
| Assay Length | 100 min |
| Target Synonyms | AST;GOT |
| Reader | Spectrophotometer |
| Test Principle | AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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