Pyruvate Dehydrogenase (PDH) Activity Assay Kit
SKU: BTL-EL-AK-02641 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K650-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Tricarboxylic Acid（TCA）Cycle |
| Sensitivity | 0.28 U /L |
| Detection Range | 0.28-168.91 U/L |
| Sample Type | serum (plasma);urine;animal tissue;cell |
| Assay Length | 15 min |
| Target Synonyms | PDH |
| Reader | Microplate reader |
| Test Principle | Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
Share Item: