NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit
SKU: BTL-EL-AK-02642 |
Brand: Elabscience
Product Description
| Cat Number | E-BC-K651-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Tricarboxylic Acid（TCA）Cycle |
| Sensitivity | 0.84 U/L |
| Detection Range | 0.84-50 U/L |
| Sample Type | animal tissue |
| Assay Length | 50 min |
| Target Synonyms | NAD-IDH |
| Reader | Microplate reader |
| Test Principle | Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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