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Phospholipase D (PLD) Activity Colorimetric Assay Kit

SKU: BTL-EL-AK-02691 | Brand: Elabscience
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Product Description

Cat Number E-BC-K783-M_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Colorimetric method
Research Field Glycolysis and Lipid Metabolism
Sensitivity 0.68 U/L
Detection Range 0.68-16.73 U/L
Sample Type Serum;Plasma;Animal and plant tissue
Assay Length 50 min
Reader Microplate reader
Test Principle Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases.
The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve.
Storage This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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