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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

SKU: BTL-EL-AK-02698 | Brand: Elabscience
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Product Description

Cat Number E-BC-K790-M_96T
Category Cell Metabolism Assay Kit
Pack Size 96T
Detection Method Colorimetric method
Research Field Glycolysis and Lipid Metabolism
Sensitivity 0.49 U/L
Detection Range 0.49-50.00 U/L
Sample Type tissue;cell
Assay Length 50 min
Target Synonyms GAPDH
Reader Microplate reader
Test Principle Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.
Storage This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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