Product Description
| Cat Number | E-BC-K853-M_48T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 48T |
| Detection Method | Colorimetric method |
| Research Field | Amino Acids and Proteins; cuproptosis |
| Sensitivity | 0.036 mmol/L |
| Detection Range | 0.036–2.0 mmol/L |
| Sample Type | serum (plasma);animal;plant tissue |
| Assay Length | 70 min |
| Target Synonyms | Gln |
| Reader | Microplate reader |
| Test Principle | Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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