Protein Construction: A DNA sequence encoding the envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Phe247-Gly675, with two amino acids "MV" at the N-terminal) was expressed with a polyhistidine tag at the C-terminus.
Accession: AAC59274.1
Expressed Host: Baculovirus-Insect Cells
Endotoxin: < 1.0 EU per ?g protein as determined by the LAL method.
Stability and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80?C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months.Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Background:
