Protein Construction:A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.
Fusion Tag C-6His
Stability and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80?C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20? for 3 months.
Formulation: LyopHilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.