TransScript® Green miRNA Two-Step qRT-PCR SuperMix
SKU: BTL-TB-MB-00299 |
Brand: Transgen Biotek
Product Description
| Cat Number | AQ202-01 |
|---|---|
| Category | Molecular Biology Product |
| Pack Size | RT system/qPCR system 20 rxns×20 μl/500 rxns×20 μl |
| Description | TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification. Highlights • Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA. • One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube. • High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data. • Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions). Applications Multiple copy and low copy gene detection. Storage at -20°C in dark for two years Shipping Dry ice (-70 ℃) |
| Storage | -80°C |
| References | 1 Yang Y, Lin Z, Han Z, et al. miR-539 activates the SAPK/JNK signaling pathway to promote ferropotosis in colorectal cancer by directly targeting TIPE[J]. Cell Death Discovery, 2021.(IF 5.24) 2 Hong Y H, Meng J, He X L, et al. Overexpression of MiR482c in tomato induces enhanced susceptibility to late blight[J]. Cells, 2019.(IF 5.65) |
| Note | The product is for research use only |
Share Item: