OZ BIOSCIENCES: Expert in Drug Delivery System

Oz Biosciences, established in 2003, develops cutting-edge transfection and transduction reagents for in-vitro and in-vivo biomolecule delivery. Oz has achieved a strong position in the field of molecular delivery systems with their innovative systems of Magnetofection™, a transfection method based on magnetic nanoparticles; Lipofection, a lipid-based transfection technology using biochemical methods; and Polyfection, a polymer-based transfection technology.

Oz’s range of reagents includes specialised tools for CRISPR, stem cell applications, cell-specific reagents, and much more. Oz also provides vectors for achieving high levels of transgene expression. With these tools, transfection and transduction efficiency and performance are greatly improved. Their aim is to assist scientists in accelerating the rhythm of discoveries by offering them technologically advanced tools such as transfection reagents and other life science research products, as well as having a specialty and expertise in drug delivery systems.

They have an extensive product range: transfection agents, proteins & plasmids, stem cell applications, mRNAs, lipid nanoparticles, assay kits, CRISPR-Cas 9, etc. for life science researchers around the world. BTL Biotechnolabs serves as an authorized Oz Biosciences distributor in India.

Transfection

• Magnetofection associates nucleic acids or other vectors with biodegradable magnetic nanoparticles coated with cationic molecules. This method is perfect for primary and hard-to-transfect cells.


• Lipofection is a lipid-based transfection technology that belongs to biochemical methods. The main advantages of lipofection are its high efficiency, its ability to transfect all types of nucleic acids in a wide range of cell types, its reproducibility, and its low toxicity.


• Polyfection, a polymer-based transfection technology with the design and synthesis of a novel patented Cationic Hydroxylated Amphipilic Multi-Block Polymer (CHAMP), is an optimised system for superior transfection efficiency in a broad spectrum of cell types with low cellular stress thanks to improved delivery mechanisms.

Proteins & Plasmids:

Optimized Cas9 Nuclease

Optimized Cas9 Nuclease S. Pyogenes is designed for genome editing in living cells or organisms and also for in vitro digestion.

R-Phycoerythrin

OZ Biosciences style="color:blue"R-phycoerythrin (R-PE) is a naturally fluorescent protein from algae and is composed of a 240KD protein covalently linked to a number of fluorophores called phycobilins. Its exceptional fluorescence characteristics make it a perfect positive control for intracellular protein delivery using OZ Biosciences Pro-DeliverIN reagent. R-phycoerythrin is provided at a 100μg/mL concentration in DPBS pH 7.4.

IgG-FITC

OZ Biosciences immunoglobulin (IgG) from goat serum has been labelled with the fluorescein isothiocyanate dye to produce a green-emitting antibody with a high fluorophore-to-protein ratio. The resulting antibody can be used as a positive control for intracellular antibody delivery with OZ Biosciences’ Ab-DeliverIN reagent.

Plasmids-pVectOZ

pVectOZ Transfection Plasmids are engineered with an optimised backbone and are ideal for all transfections. OZ Biosciences proposes five expression vectors encoding for the most popular reporter genes (CAT, GFP, LacZ, Luciferase, and SEAP).

pVectOZ-encoding genes:

• Chloramphenicol Acetyltransferase (CAT)—pVectOZ-CAT
• Green Fluorescent Protein (GFP)—pVectOZ-GFP
• beta-galactosidase (Lac Z)—pVectOZ-LACZ
• Luciferase (Luc): pVectOZ-LUC
• Secreted Alcaline Phosphatase (SEAP)—pVectOZ-SEAP

The transgene expression level depends mainly on the promoter, enhancer, terminator, and plasmid backbone. The pVectOZ expression cassette was designed to express transgene products in many mammalian cells and tissues. This vector has been modified to eliminate sequences affecting transgene expression levels while optimising those critical for high levels of expression. The final expression cassette accommodates the high levels of transgene expression in mammalian cells as well as the high yield of plasmid production in Escherichia coli. The resulting plasmid is the ideal vector for expression in vitro and in vivo.

pVectOZ Features:

• Highest levels of transgene expression in mammalian cells and tissues
• Suitable for all transfection applications (in vivo & in vitro)
• High copy number production in Escherichia coli
• LPS-endotoxin free, supercoiled and highly purified: transfection grade approved

STEM CELL APPLICATIONS:

ViroMICST

Viro-MICST Transduction Reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on the i-MICST™ technology (integrated magnetic immuno-cell sorting and transfection/transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system. For efficient and selective gene delivery to target cells, Viro-MICST can be used in combination with any viral vector and allows you to reduce cell manipulation steps and save time and material.

• Highly efficient
• Rapid, simple, and ready to use
• Suitable for all cells

ViroMag

ViroMag Transduction Reagent is a magnetic nanoparticle formulation dedicated to increasing virus infection and transduction capacities. It is suitable for all viruses. Based on MagnetofectionTM technology, this reagent allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement in virus transduction with extremely low vector doses. Due to its specific properties, ViroMag is ideal for infecting non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control, and assist viral transductions, so that no molecular biology processes or biochemical modifications are required.

Key Features:

• Increases transduction efficiency
• Accelerates the transduction process
• Efficient for hard-to-infect and non-permissive cells
• Concentrates the viral dose onto the cells
• Suitable for all type of virus
• Synchronise cells adsorption/infection without modifications of the viruses

si3D-Fect

si3D-Fect™ is a 3D transfection reagent specifically designed and developed for silencing gene expression in cells cultured on 3D Scaffolds.

3D matrices not only add a third dimension to the cells’ environment, but they also allow for significant differences in cellular phenotype and behavior. In this way, 3D matrices bearing complexes formed with si3D-Fect™ reagent and siRNA are colonised by cells to be transfected in a more natural environment. si3D-Fect™ reagent associated with 3D matrices allows numerous cell transfections in order to study tissue engineering, tissue regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation, colonization, and neurite growth.

Key Features

• Highly efficient for gene silencing in 3D matrices
• Ideal for any 3D scaffolds (sponges, matrices, inserts)
• Dedicated to short nucleic acid sequences (siRNA, miRNA…)
• Long term gene silencing
• Universal (primary cells and cell lines)
• Serum Compatible

Senescence Kit

Senescence Kit is ideal when cells in culture show abnormalities typical of the Hayflick limit, after a long period of normal growth. The cells vary in size and shape, and the cytoplasm begins to be granular with many cell inclusions and debris formed in the medium. Senescence goes with a decrease in proliferation potential, telomere shortening, and impairment of cell function (i.e., a decrease in the differentiation potential of stem cells). During senescence in mammalian cells, an endogenous lysosomal b-galactosidase is overexpressed and is accumulated within the ageing cells. The presence of this b-gal activity is a marker of an ageing cell population in vitro.

RmesFect™ Stem Transfection Reagent is based on TEE technology and specifically designed for mRNA transfection in stem cells with high efficiency and low toxicity. mRNA transfection provides several advantages over plasmid DNA delivery:

• There is no need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.
• Faster protein expression than DNA transfection
• No genomic integration
• Protein expression in a total promoter independent manner

Pro-DeliverIN™ Transfection Reagent is an innovative reagent allowing intracellular delivery of biologically active proteins. This lipid-based formulation is the first serum-compatible reagent to deliver functional proteins into living cells. The proteins delivered inside cells retain their biological functions.

Key Features:

• Intracellular delivery of functionally active proteins
• Highly efficient in many primary cells and cell lines
• Serum compatible
• Biodegradable and non-toxic
• Easy: straightforward protocol and ready-to-use (no chemical conjugation required).

Ab-DeliverIN™ Transfection Reagent is the first reagent dedicated to the intracellular delivery of fully functional antibodies. Ab-DeliverIN opens up new fields of investigation in proteomics, for instance, to elucidate complex molecular mechanisms or to design new potential therapies.

Key Features:

• Intracellular delivery of functionally active proteins
• Highly efficient in many primary cells and cell lines
• Serum compatible
• Biodegradable and non-toxic
• Straightforward protocol and ready-to-use reagents

AdenoMag

AdenoMag Transfection Reagent is a magnetic nanoparticle formulation specifically dedicated to enhance Adenovirus and Adeno-Associated Virus (AAV) infection and transduction capacities. Based on Magnetofection technology, it allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement in virus infectivity with extremely low vector doses. Due to its specific properties, AdenoMag is applicable to all adenoviral and AAV vectors and is ideal for infecting non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control, and assist adenoviral and AAV transductions in such a way that no molecular biology processes or biochemical modifications are required.

Key Features:

• Increase transduction efficiency
• Accelerate the transduction process
• Efficient for hard-to-infect and non-permissive cells
• Concentrate the viral dose onto the cells
• Synchronise cells adsorption/infection without modifications of the viruses

D-Fect

3D-Fect™ is suitable for all kinds of scaffolds and cells. This 3D Transfection Reagent is specifically developed to directly transfect cells cultured in 3D scaffolds. This reagent is based on a novel technology that adds a third dimension to cell culture and transfection. 3D matrices not only add a third dimension to cells’ environments, but they also allow for creating significant differences in cellular characteristics and behaviours.

Because 3D matrices are routinely used in basic research and therapeutic applications, OZ Biosciences has developed the 3D-Fect™ Transfection Reagent specifically for 3D-scaffolds. In this way, scaffold-based 3D matrices activated with 3D-Fect™ /DNA complexes are colonised by cells, which are subsequently transfected in situ and in a more natural environment. This method allows studying tissue engineering and regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation, colonization, and neurite growth.

Lullaby Stem

Lullaby Stem siRNA Transfection Reagent is ideal for gene silencing in stem cells. Lullaby Stem enables siRNA and miRNA transfection of multipotent and embryonic stem cells with high efficiency and very low toxicity. According to TEE technology, its lipid-specific formulation protects small RNA from extracellular degradation, transports them across cell membranes, and efficiently releases them into stem cell cytoplasm.

Key Features:

• High gene silencing efficiency of multipotent and embryonic stem cells
• Minimized toxicity due to reagent biodegradability and the low siRNA/miRNA amount required
• Reliable and reproducible gene knockdown results
• Serum and antibiotics are compatible.

ViroMICST Stem Transduction Enhancer

Viro-MICST™ Stem Transduction Enhancer is a new specific magnetic nanoparticle formulation issued from our Magnetofection™ technology. Its properties were specifically designed for stem cell lentiviral transduction in order to achieve a high infection rate directly on magnetic cell sorting devices (column or magnet). As a stabilised magnetic nanoparticle formulation, ViroMICST Stem offers a simple and reproducible method for increasing lentiviral infection and transduction of difficult cell types such as CD34+ hematopoietic stem cells, both cell lines and primary cells, during cell sorting. Due to the total biodegradability of the magnetic nanoparticles, ViroMICST Stem is non-toxic and ideal for CD34+ hematopoietic stem cell transduction.

This technology combines cell isolation and genetic modification in one simple, efficient, and reliable integrated system. ViroMICST Stem Transduction Reagent allows genetically modifying cells directly on magnetic cell purification columns. Based on the i-MICSTTM technology (integrated magnetic immuno-cell sorting and transfection/transduction), this technology combines cell isolation and genetic modification in one simple and reliable integrated system.

Key Features:

• Increased Lentiviral Infection
• Reduce cell manipulation steps and save time and material
• Designed for stem cells

mRNAs

OZ Biosciences offers mRNAs that mimic fully processed, mature mRNAs. These mRNAs are stabilised with a 5’ Cap 1 structure and a 3’ poly(A) tail and are optimised to yield improved stability and performance. They are modified with 5-methoxyuridine (5-moU, where 5-moU replaces U) or N1-methyl-pseudouridine (N1-m) to reduce innate immune responses. We also offer unmodified mRNAs.

5 categories of ready-to-use mRNAs

• Reporter gene mRNAs: ideal controls to study transfection efficiency
• Genome editing mRNAs: Cas9 mRNA for CRISPR genome editing
• Vaccine mRNAs are ideal as controls for immunisation or vaccine studies.
• Gene Replacement mRNAs: EPO mRNA
• Fluorescent mRNAs: Cy5 GFP mRNA, Cy5 GFP mRNA

Custom mRNA Synthesis Service is also available.

With over 15 years of nucleic acid delivery expertise, OZ Biosciences is confident in providing you with the best-in-class service for the production of high-quality mRNAs. They are able to synthesise custom mRNAs at microgram to multigram scales, from a few hundred up to several thousand bases, with a wide variety of modifications, including 5’ terminal modifications with caps, internal modifications such as 5-methoxyuridine (other modified nucleotides are available), and 3’ modifications such as poly-A tail. fluorescent labelling with Cy5, Cy3, or other options is available.

Lipid Nanoparticles (LNP)

OZ Biosciences has developed a Microfluidics Platform for the reproducible development of safe and potent drug delivery vehicles for pharmaceutical applications.

We can support every stage of your mRNA-LNP production, from mRNA synthesis to LNP formulation development, manufacturing, and fill & finish.

For RNA, DNA, or API encapsulation, you can provide us with your molecule of interest and we will formulate it into LNPs.

Aside from our custom service, we also offer ready-to-use NanOZ-LNP and mRNA products for your research studies:

• NANOZ LNP-mRNA(GFP)
• NANOZ LNP-mRNA(Luc)

Luciferase Assay kit

The luciferase assay kit is the most sensitive analytical tool for measuring gene expression. Fast and easy, accurate and linear, this kit detects and quantify firefly luciferase in transfected eukaryotic cells reaching high sensitivity level. Since the light emission is constant (>15 min) when using this kit, there is no need to use an automated luminometer for substrate injection. The emitted light is recorded by a standard luminometer at 560nm. Luciferase assay kit contains optimized components (cell lysis buffer, luciferase substrate and Luciferase Assay Buffer) to ensure maximal sensitivity with a consistent light output.

Key Features:

• Very sensitive and quantitative assay with a wide dynamic range
• Bioluminescence measurements are performed directly into transfected wells.
• Cell lysis buffer is optimized for Luciferase assay buffer to ensure maximal sensitivity
• Light emission is proportional to number of luciferase enzyme molecule

CPRG kit

OZ Biosciences CPRG assay kit is ideal to quantify low expression level of beta-Galactosidase. LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, b-Gal), is very stable, resistant to proteolytic degradation and easily assayed. The levels of active beta-Gal actosidase expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-b-D-galactopyranoside), substrate to a dark red product.

The high sensitivity of this substrate improves the measurement of beta-Galactosidase activity when the reporter gene expression is low. (Absorbance at 570-595 nm). All the necessary reagents are provided in this assay kit and offer a rapid, simple, and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells. BTL Biotechnolabs, based in India, proudly serves as an Oz Biosciences supplier in India.

Key Features:

• Convenient for all transfection assays
• Suitable for cultured cells and tissues.
• Simple, rapid and easy-to-use
• Economical
• Optimized with stable buffers

FluoProdige Assay Kit

The FluoProdige Protein Quantification Assay Kit presents a complete fluorometric assay for protein and peptide quantification. FluoProdige facilitates and improves protein quantification by providing sensitivity and convenience when compared to classic colorimetric measurements such as Lowry or BCA.

Applications:

• Accurate and highly sensitive

Detect as little as 40 ng/mL; large linear detection range: over 3 orders of magnitude

• Simple & Fast

Protocol: 15 minutes; photo of signal within 30 minutes; signal duration: up to 4 hours

• Safe & Biodegradable Natural Product: Non-Toxic, Heavy Metal Free
• compatible with many buffers

Robust to interfering compounds such as DNA, detergents, DTT, and urea. This assay kit uses a stable analogue to the epicocconone molecule that reversibly binds to lysine, arginine, and histidine residues in proteins and peptides to yield an intensely fluorescent product. The fluorescent signal (518/605) is directly proportional to protein amount among a wide range of protein concentrations, rendering this kit highly sensitive. When bound to proteins, excitation and emission signals are 518 and 605 nm, respectively.

OZ Blue Cell Viability Kit

The OZ Blue Cell Viability Kit is a ready-to-use assay system based on fluorimetric or colorimetric quantification of metabolic activity in living cells. The Resazurin dye is used as an indicator of cell viability.

Main Features:

• Non-toxic: Allows additional analysis of proliferating cells (mRNA, cytogenetics, apoptosis, immunophenotyping, etc.) and continuous monitoring of cultures over time.
• High Sensitivity: More sensitive than [3H] Thymidine incorporation and tetrazolium reduction assay (MTT, XTT)
• Simple: a single, ready-to-use reagent. There is no need for washing or extraction procedures.
• Versatile: can be used on cell lines, primary cells, bacteria, and fungi
• Flexible: Colorimetric and fluorescence detection allow compatibility with flow cytometry, plate readers, and microscopy detection.
• Rapid and cost-effective

CRISPR/CAS9 Transfection Reagents

RmesFect CRISPR

RmesFect™ CRISPR transfection reagent based on TEE technology is specifically designed for mRNA and gRNA transfection with high efficiency and low toxicity. RmesFect™ is efficient in a large variety of cells.

Benefits:

• Highly efficient: achieve RNA delivery in all cells
• Ready-to-use: no need for additional buffers
• Low nucleic acid amount: minimised toxicity
• Compatible with any cultural medium
• protects RNAs against degradation
• Compatible with any cultural medium
• Serum compatible: Medium change is not required

ProDeliverIN CRISPR Kit is a transfection reagent optimised for recombinant Cas9 protein delivery or Cas9/gRNA RNP complexes. For your gene editing applications, this reagent provides high transfection efficiency with minimal toxicity.

Benefits of Cas9 Protein Over Cas9 DNA: The Cas9 recombinant protein is delivered more rapidly than nucleic acids and is fully active once inside the cells without a latency period (in contrast to the transcription and translation machinery required for the nucleic acids). As for mRNA, it presents the advantages of avoiding genomic integration and being cell cycle independent.

Key Features:

• High-efficiency delivery of Cas9 protein or Cas9 gRNA complexes
• Low cell toxicity - enable to start your experiment with less cells

Additional products for CRISPR-Cas9 experiments:

• Polymag CRISPR for genome editing using expression plasmids
• RmesFect CRISPR for mRNA transfection
• ViroMag CRISPR to enhance the transduction efficiency of CRISPR/Cas9 viruses

BTL Biotechno Labs Pvt Ltd. is an authorized OZ Biosciences Channel partner in India, providing the scientific and pharmaceutical community with comprehensive access to the product suite of Oz Biosciences that encompasses a wide range of innovative solutions such as transfection agents, proteins & plasmids, stem cell applications, mRNAs, lipid nanoparticles, assay kits, CRISPR-Cas 9 and custom services. Our partnership enables us to offer cutting-edge solutions that meet the unique needs of researchers and industry professionals throughout the region. We now serve as a trusted supplier of Oz Biosciences products in India.