Can I use cell lysates with the strips or arrays?

Cell lysates should not be used with these products because they increase background noise and potentially mask positive results due to non-specific binding of other cellular elements or proteins.

I don't see the binding pattern I expect.

A complete lack of signal (white membrane) is often caused by high detergent concentrations in the assay solution or a loss of binding protein activity. In all scenarios, it is advised to carry out a positive control for the target lipid to identify the problem. If abnormal binding is detected, the cause could possibly be severe detergent conditions or excessive membrane formation following the application of the detection reagent.

Is there a recommended detection reagent?

All strips and arrays can be utilized with precipitating agents or chemiluminescent solutions after being internally validated using secondary antibodies that have been HRP-conjugated. These products allow the use of fluorescently conjugated secondary antibodies, although we advise the user to maximise the antibody concentration in order to reduce background signal.

How can I reduce a high background signal?

High background is most likely caused by excessive binding protein or insufficient cleaning during the initial incubation stage. It is also possible to lessen background noise by changing the blocking buffer's makeup.

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Range of ELISA Kits, Antibodies, Biochemicals, Recombinant Proteins & Assay Kits

Echleon Biosciences Distrubutor in India

By: Team BTL Biotechno Labs Pvt. Ltd.

Echleon biosciences is a global leader in supplying biochemical reagents, assays, and screening services to advance academic research and pharmaceutical development. The company is engaged in the study of lipid cell signalling and critical metabolic enzymes, which provide therapeutic targets for the drug development and diagnostics for various diseases including cancer, inflammation, diabetes, infections and cardiovascular disease. The aim of the company is to expertise in custom synthesis and assay development for creating the best reagents for biological and drug discovery research. BTL Biotechno Labs Pvt. Ltd. is representing as an authorized Echleon Biosciences distributor in India exclusively to support research in similar fields.

Lipid-Protein Interaction Tools

Lipid-protein interaction tools lower the barriers for interrogating lipid-protein binding partners. Current advancements in lipidomic and proteomic technology have led to progress in searching for protein-lipid interactions in nuclear extracts, cell lysates, and other related cellular components. For this purpose, we provide certain tools like:

Lipid Strips
Lipid Beads
PolyPIPosomes
Lipid Plates

Lipid Antibodies

Lipid antibodies (Abs) that have been validated in a range of applications including ICC and IHC.

Mouse Anti-PI(4)P Antibody
Mouse Anti-PI(4,5)P2 Antibody
Mouse Anti-PI(3,4,5)P3 Antibody
Lysophosphatidic Acid (LPA) Antibody
Sphingosine 1-Phosphate (S1P) Antibody

Visit our range of antibodies products to order online.

BTL Biotechno Labs Pvt. Ltd. is a Echleon Biosciences distributor in Delhi, India based recognized distributor of Echelon Biosciences in the field of antibodies products. We are committed to position the most reliable supplier of echelon biosciences products in the entire Indian market.

Nanobodies

There is a recent development in the field of antibodies: unexpected discovery of heavy chain-only antibodies (HcAbs) in sharks and camelids. Generally an Abcontains two 50 kDa heavy chains and two 25 kDa light chains for a combined molecular weight of 150 kDa where as camelid HcAbs are made up of two single variable domain heavy chains only which gives them size smaller than conventional antibodies. Importantly, the single variable domain (VHH) from HcAbs can be isolated and maintains the binding properties observed with the full HcAbs.

The resulting VHH antibodies were named ‘nanobodies’ because of their low molecular weight of ~15 kDa. As with conventional Abs, nanobodies can generally be utilized for long-standing methods such as western blotting, ELISAs (ELISA Kit Distributor), and immunofluorescence. Further nanobodies have significant advantages in cellular and in vivo imaging. Due to their small size, they become an excellent tool for super-resolution microscopy and also provide the advantage of binding to cavity-like regions of proteins. Unlike conventional Abs, the antigen-binding region of nanobodies tends to have a longer, protruding shape.

Lipid Nanoparticle

RNA-based therapeutics have been under development for over 30 years however, the COVID-19 pandemic has rapidly accelerated their development, chiefly with mRNA-based vaccines against the SARS-Cov2 virus which has involved the use of lipid nanoparticles (LNPs) for delivering deliver RNA.

Naked RNA cannot be take-up by the cells as it is immunogenic and susceptible to enzymatic degradation. Hence to overcome these problems, LNP has been used as a delivery vehicle for RNA which allows it to enter the cells and further protects it from enzymatic degradation and then release the contents into the cytoplasm which will lead to synthesis of desired protein.

ALC-0159
ALC-0315
cKK-E12
SM-102

PIP Mass Assays

Phosphatidylinositol phosphates (PIPs) are more than just membrane components IP signaling has also emerged as an important target in cancer therapy, hence, there is a need to better understand the involvement of PIPs in cellular processes.

PIP3 Mass Assay
PI(4)P Mass Assay
PI(3,4)P2 Mass Assay
PI(4,5)P2 Mass Assay

Hyaluronic acid

Hyaluronic Acid (HA) is the chief component which participates in cell adhesion and cell-to-cell communication and is an excellent target for biomedical applications. HA has been implicated in the development of cancer and cardiovascular disease. Injectable HA is already widely used to treat osteoarthritis.

Many researchers are now studying HA-based scaffolds, wound healing, tissue engineering, and drug delivery applications. This kind of research often involves chemical modification of HA to either extend the half-life or accelerate the degradation of HA applied to tissues. Measuring the amount of HA or the activity of relevant HA synthases or hyaluronidases can be used to monitor the efficiency of the modification. Further, using high-quality medical grade HA with lot-to-lot constancy as the base of these applications would save researchers’ time.