Svar Life Science: Complement System Solutions

The complement system is an integral part of the innate immune response. Given its important role in many human disorders, complement activation studies are important in a wide range of applications – from drug development and medical device safety to clinical diagnostics and basic research. There are three pathways of complement activation: the classical pathway, which is triggered directly by the pathogen or indirectly by antibody binding to the pathogen surface; the MB-lectin pathway; and the alternative pathway, which also provides an amplification loop for the other two pathways.

At the basic level, the broad functions of the complement system can be split into three areas: (1) the activation of inflammation; (2) the opsonization (labeling) of pathogens and cells for clearance/ destruction; and (3) the direct killing of target cells/microbes by lysis. C3 and C4 are the most commonly measured complement components. A complement test may be used to monitor people with an autoimmune disorder. It is done to see if treatment for their condition is working. When the complement system is turned on during inflammation, levels of complement proteins may go down.

Svar has over 15 years’ experience in complement system assay development. Their complement system assays have been developed by experienced scientists in close collaboration with key opinion leaders, to yield sensitive, reliable, and easy-to-use products for the exploration of most aspects of the complement system.

Svar offers functional and biomarker assays that can be used to explore every angle of complement system-involvement in a wide range of application areas.

• Reliable, objective, and easy-to-interpret results
• Results in just a few hours – optimise time usage with Svar's biomarker and functional complement assays
• Low inter-assay variability and excellent reproducibility
• Based on the well-established and flexible ELISA and reporter gene technology
• Developed by their in-house experts in collaboration with key opinion leaders
• Ready-to-use reagents for a streamlined workflow
• Excellent specificity means clear results without false positives
• Only company to offer exploration of all three complement system pathways individually
• Increase the resolution of amplification loop research with our novel functional Factor P assays

Based on the well-established and robust ELISA technology, our products can be used in standard setups as well as automated setups with ELISA robots. Ready-to-use reagents contribute to the ease-of-use of the assays. Svar’s complement activity biomarker assays offer excellent specificity, which means that you get easy-to-interpret data without having to worry about false positives. In addition, our assays offer low batch-to-batch variation compared to hemolytic methods, where erythrocytes can differ between lots, which means more consistent and robust results. Taken together, one can get reliable and objective results in as little as 2–4.5 hours, drastically reducing time spent compared to hemolytic assays.

Product Categories

Biomarker assays

Ideal for the detection of complement activation or dysregulation in the complement system.

Functional assays

Perform analyses of all three pathways to get the full picture of the complement system, or zoom in on the amplification loop with Factor P functional assay.

Cell-based assays

iLite® cell-based functional reporter-gene assay in an assay-ready format. Enables a fast, streamlined work process with low variability.

Flexi Solutions

Complement products for flexible assay setup on automated platforms of your choice.

Functional Assessment

The Svar functional complement assays under the Wieslab® brand were developed together with a network of global key opinion leaders (KOLs) in 2005 and have since been used to answer the need for an easy-to-use method of detecting complement deficiencies and to explore the effect of complement targeting drugs. The Alternative Pathway amplification loop is central to the essential amplification of the complement response. The novel, Svar Factor P functional assay has been developed to enable analysis of the complement up-regulator, Properdin, and its associated C3-convertase function.

The Svar complement functional assay solution is an ELISA-based platform that enables the determination of specific activity of all three pathways separately without interference from the others. The well-established ELISA technology means a robust and standardised assay platform, delivering fast, reliable, and objective results that are easy to interpret within as little as 3 hours.

Functional complement assays

Complement C4d [COMPL C4d RUO]
Complement TCC [COMPL TCC RUO]
Complement Factor P Quantitative Assay [COMPL FPQ RUO]
iLite® C5a Assay Ready Cells [BM4075]
Complement Flexi Diluent [FX1344]

C4d

Complement C4d Flexi Capture antibody [FX1342]
Complement C4d Flexi Calibrator stock [FX1345]
Complement C4d Flexi Detection antibody [FX1343]
Complement C4d Flexi Low control [FX1346]
Complement C4d Flexi High control [FX1347]

TCC

Complement TCC Flexi Capture antibody [FX1354]
Complement TCC Flexi Calibrator stock [FX1356]
Complement TCC Flexi Low control [FX1357]
Complement TCC Flexi High control [FX1358]
Complement TCC Flexi Detection antibody [FX1355]
WIESLAB® Complement Alternative Pathway [COMPLAP330]
WIESLAB® Complement Alternative Pathway (RUO) [COMPLAP330RUO]
WIESLAB® Complement Classical Pathway [COMPLCP310]
WIESLAB® Complement Classical Pathway (RUO) [COMPLCP310RUO]
WIESLAB® Complement MBL Pathway [COMPLMP320]
WIESLAB® Complement MBL Pathway (RUO) [COMPLMP320RUO]
WIESLAB® Complement Screen [COMPL300]
WIESLAB® Complement Screen (RUO) [COMPL300RUO]
Complement Factor P Functional Assay [COMPL FPF RUO]

cell-based complement assays offer a new way of studying the complement system. Based on iLite® technology, these assays use a reporter gene system to detect the presence of the complement component for which they were designed. iLite assays offer excellent sensitivity and specificity which ensures that you get accurate results. As an added convenience, all iLite cell lines come assay-ready – no cell culturing is required. This results in a faster workflow and dramatically improved reproducibility and overall performance.

iLite cells express receptors on their cell surfaces that are specific for the complement component for which they were designed. When a complement component binds to its receptor, an intracellular signaling cascade is initiated which will result in the expression of luciferase. The amount of luciferase generated can be used to calculate the amount of the complement component in the sample. Many iLite assays also include a second constitutively expressed luciferase gene which can be used for normalization.

The purpose of the Complement TCC Flexi reagents is to specifically detect the soluble terminal complement complex (TCC, also known as sTCC or sC5b-9) which is an end product of complement activation of all three complement pathways. The Complement TCC Flexi application note and reagents enables users to independently set up, customize and optimize a Complement TCC Flexi assay based on Svar Life Science’s Complement TCC assay on Gyrolab® (Gyros Protein Technologies) automated platforms or other platforms of their choice.

Cell Based Complement Assays

Complement Flexi- for Automated Platforms

The Svar Life Science Complement Flexi Solutions allow users to independently set up, customize and optimize assays based on existing Svar Life Science assays. In collaboration with Gyros Protein Technologies, Svar has developed application notes for use of the Complement Flexi Solutions on the automated Gyrolab® platforms. The Flexi Solutions can of course also be used in manual assays as well as for other laboratory techniques, such as immunoblotting and immunohistochemistry, where your imagination is the only limit. BTL Biotechno Labs, as a trusted distributor, plays a pivotal role in bringing Svar Life Science Solutions in India.

It is recommended to set up Complement TCC Flexi assays as 3-step sandwich assays with the capture and detection antibodies used in their designated functions to ensure optimal assay performance.

Complement TCC Flexi reagents

Complement TCC Flexi Capture antibody
Complement TCC Flexi Detection antibody
Complement Flexi Diluent
Complement TCC Flexi Calibrator stock
Complement TCC Flexi Low control
Complement TCC Flexi High control

Complement C4d Flexi reagents

Complement C4d Flexi Capture antibody
Complement C4d Flexi Detection antibody
Complement C4d Flexi Diluent
Complement C4d Flexi Calibrator stock
Complement C4d Flexi Low control
Complement C4d Flexi High control

Complement Activity Biomarkers

The Svar Complement activity biomarkers give valuable intelligence in several situations where one might suspect that complement activation plays a role in the disorder. The Svar assays for complement activity biomarkers are developed to target the unique neoepitopes only presented at the complement component or complement activated.

The Svar Complement C4d , Complement TCC, and Complement Factor P assays are flexible and easy to use enzyme immunoassays with ready to use reagents and short incubation times leading to reduced hands-on-time for the user.

Complement TCC

The Terminal Complement Complex, or TCC, is a product of the terminal pathway and thus a result of activation of any of the three complement activation pathways. As a reflection of total in vivo complement activity, increased levels of TCC can be detected in several acute, flaring, and chronic diseases.

Complement C4d

C4d is a marker for complement activity originating from the classical or lectin pathways. It is an established histological biomarker with a strong association to antibody mediated rejection of grafts. Moreover, C4d has increasingly been used as a biomarker for diagnostic and prognostic purposes, in conditions ranging antibody-mediated disease to cancers.

Complement Factor P

Factor P, also known as Properdin, is the only known positive regulator of alternative pathways of complement response and the key to ensuring sufficient complement activity. Properdin imbalance or dysfunction has been linked to several health disorders, and assessing its prevalence is a useful tool for a better understanding of complement regulation. In addition to the quantitative Factor P assay linked below, we also offer a functional Factor P assay.

Complement Testing Application:

• Help diagnose the cause of recurrent microbial infections (such as Streptococcus pneumoniae, Neisseria meningitides, and Neisseria gonorrhoea), angioedema, or inflammation.
• Help diagnose and monitor the activity and treatment of autoimmune diseases and immune complex-related conditions.
• Evaluate new complement-targeted therapies (whether the aim is to inhibit or enhance complement functionality), determine efficacy, and potency of complement-targeted drugs.

We, BTL Biotechno Labs Pvt. Ltd., as a Svar Life Science channel partner in India, provide their complete range of complement system solutions, including ELISA kits, antibodies, cell-based assays, biomarkers, cell lines, and many more, to the Indian scientific community to support immunological research.