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PIP Strips - Lipid-Protein Interaction Assay
PIP strips are membranes pre-spotted with all eight phosphoinositides, seven other biologically important lipids, and a blank control. PIP strips are used in a simple protein-lipid overlay assay to help determine the specificity of your protein towards one or more of these lipids. Briefly, once the membrane is blocked, it is incubated with the protein of interest. The membrane is then washed and incubated with an HRP-labeled antibody against the protein or your protein's affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualised with the appropriate equipment based on the detection reagent used.
BTL Biotechno Labs - Distributor of Echelon Bioscience in India
We, BTL Biotechno Labs Pvt. Ltd., a distributor of Echelon Bioscience in India provide their complete range of PIP Strips having 2 x 6 cm hydrophobic membranes with 100 pmol of the lipids:
Lysophosphatidic acid (LPA), Lysophosphocholine (LPC), Phosphatidylinositol (PtdIns), Phosphatidylinositol (3)-phosphate(PtdIns(3)P), Phosphatidylinositol (4)-phosphate (PtdIns(4)P), Phosphatidylinositol (5)-phosphate (PtdIns(5)P), Phosphatidylethanolamine (PE), Phosphatidylcholine (PC), Sphingosine 1-Phosphate (S1P), Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2 ), Phosphatidylinositol (3,5)-bisphosphate (PtdIns(3,5)P2), Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P2), Phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), Phosphatidic acid (PA), Phosphatidylserine (PS).