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3,3′,5′-tetramethylbenzidine, or TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and fluorescent lights. TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase. The resulting one-electron oxidation product is a diamine-diamine complex, which causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at the wavelengths of 370 and 650 nm. The reaction can be halted by the addition of acid or another stop reagent. Using sulfuric acid turns TMB yellow, with a peak absorbance of 450 nm. The amount of converted TMB may be indexed by the amount of 450 nm light it absorbs.

BTL Biotechno Labs Pvt. Ltd. is formed with the aim of serving and supporting the Indian scientific community, CROs, and pharmaceutical companies and being their technology and knowledge partners by providing them with the highest quality life science research products. We are the leading supplier of TMB substrate in India at the best price. TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as a visualisation reagent used in enzyme-linked immunosorbent assays (ELISA).
For more details, please contact us @ BTL Biotechno Labs | Life Science Research Products suppliers in India (


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