Product Description
| Cat Number | 66305-3 |
|---|---|
| Category | Biochemical |
| Pack Size | 250g |
| Description | Methylene Blue is a recommended stain for Agarose Gels when recovery of DNA is required. The benefit of using methylene blue over ethidium bromide is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box in place of UV light box eliminates mutations that may affect the integrity of the isolated DNA. For DNA Staining: Stain a 1% Agarose gel with DNA for 15 min with 0.02% Methylene Blue solution in distilled water and Destain with distilled water for 15 minutes. For RNA Staining: Soak the RNA bonded to nylon or nitrocellulose dried membrane in 5% acetic acid at room temperature for 15 minutes. Then, place the filter in 0.5 M sodium acetate (pH 5.2) and 0.04% methylene blue for 5 – 10 minutes at room temperature. To destain, rinse the filter in water for 5 – 10 minutes. Detailed protocol is provided with the product. |
| Research Field | Biochemicals/Chemicals |
| Note | The product is for research use only |
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