Product Description
| Cat Number | 66315-0 |
|---|---|
| Category | Biochemical |
| Pack Size | 6 mL x 4 components |
| Description | DAB (3,3'Diaminobenzidine) is a commonly used chromogen used in immunohistochemical and immunoblotting applications. DAB reacts in the presence of HRP / peroxidase enzyme to generate a brown colored precipitate bearing optimum contrast with pigmented tissues and counterstains at locations where peroxidase-conjugated antibodies are bound to samples, that is insoluble in alcohol and xylene. DAB chromogen demonstrates equivalent efficiency when utilized as a single or multiple antigen label and offers heat-resistance feature for suitable incorporation in double labeling techniques such as in situ hybridization and immunohistochemistry. Our DAB kit is supplied with refrigerator stable 20X concentrates of 1% DAB substrate, 0.3% hydrogen peroxide solution, and 0.01M phosphate buffer. It also includes a 1% nickel ammonium sulfate solution that provides an option to the researcher for adjusting the reaction product color from brown to more intense gray/black facilitating better visualization with an appropriate choice of counterstain. The resulting precipitate is insoluble in major organic solvents involved in the staining process, thus making it an ideal substrate for immunostaining and other blot-based protein applications including other counterstains and mounting media (aqueous or non-aqueous mounts). Features: • Routinely used for IHC/ICC/ISH and blots • Heat Stable and provides permanent mounting • Ideal for single and multiple antigen labeling • Compatible with aqueous or non-aqueous mounting media Form: Liquid Storage: 4°C |
| Research Field | IHC/Immunohistochemistry |
| Storage | 4 Degrees C |
| Note | The product is for research use only |
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