Product Description
| Cat Number | 66326-0 |
|---|---|
| Category | Biochemical |
| Pack Size | 100 mL |
| Description | Fixatives such as formalin or other such aldehyde-based agents readily activate intrinsic protein crosslinking responsible for masking the cellular, membrane and nuclear antigenic sites in tissue samples that lead to a weak or suppressed signal/stain during immunohistochemical analysis. Citrate buffers are commonly incorporated in antigen detection by breaking the protein crosslinks between antigens and substances in the fixation medium and thereby revealing the antigens/epitopes in formalin-fixed and paraffin embedded tissue sections, in turn enhancing the staining intensity of antibodies. These buffers are also routinely used for RNA isolation, primarily due to their ability to prevent base hydrolysis. Sodium Citrate buffer is utilized as a heat-induced antigen retriever for formalin-fixed paraffin-embedded (FFPE) tissue sections prior to incubation with antibodies or DNA fluorescent in situ hybridization. The use of Sodium Citrate buffer, pH 6.0 on such tissue sections greatly enhances the accessibility of antibodies to tissue antigens, thus improving the resulting detection signal. Applications: • Antigen unmasking during immunohistochemistry and other imaging procedures • Used in acid phosphatase reactions in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate • Enhances staining intensity without contributing to the non-specific background signal in immunofluorescence CAS: 6132-04-3, 68-04-2 Synonyms: trisodium citrate dihydrate, sodium citrate dihydrate, sodium citrate tribasic dihydrate Molecular Formula: C6H9Na3O9 Molecular Weight: 294.10 g/mol InChI Key: NLJMYIDDQXHKNR-UHFFFAOYSA-K |
| Research Field | IHC/Immunohistochemistry |
| Storage | Ambient |
| Note | The product is for research use only |
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