Product Description
| Cat Number | BST-MM101 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Mouse BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser286. |
| Target | BST1 |
| Host | Mouse |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism. |
| Molecular Mass | The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Ala25-Ser286 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q64277 |
| Amino Acid Sequence | Recombinant Mouse BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Ser286. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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