Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)
SKU: BTL-KB-P-00416 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | BTN-HM123 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail. |
| Target | BTN2A1&BTN3A1 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells. |
| Molecular Mass | The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa and 33-38 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q7KYR7-1(BTN2A1)&O00481-1(BTN3A1) |
| Amino Acid Sequence | Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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