Human CALCA/CGRP Protein (CAL-HM201)
SKU: BTL-KB-P-00442 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | CAL-HM201 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala26-Asn141. |
| Target | CALCA/CGRP |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
| Scientific Background | Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca. |
| Molecular Mass | The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Ala26-Asn141 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | NP_001732.1 |
| Amino Acid Sequence | Recombinant Human CALCA/CGRP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala26-Asn141. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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