Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)
SKU: BTL-KB-P-00681 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | CD3-HM505B |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus of CD3E and hFc (IgG1) tag at the C-Terminus of CD3D.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D). |
| Target | CD3E&CD3D/CD3 epsilon&CD3 delta |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Molecular Mass | The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P07766(CD3E)&P04234(CD3D) |
| Amino Acid Sequence | Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus of CD3E and hFc (IgG1) tag at the C-Terminus of CD3D.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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