Mouse CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-MM102)
SKU: BTL-KB-P-00683 |
Host: Mouse |
Brand: Kactus Bio
Product Description
| Cat Number | CD3-MM102 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Mouse CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed as a heterodimer of CD3E and CD3G by co-expression in HEK293. Subunit CD3E is fused with a Flag tag at the C-terminus and subunit CD3G is fused with a His tag at the C-terminus. It contains Asp22-Asp108 (CD3E) and Gln23-Ser116 (CD3G). |
| Target | CD3E&CD3G/CD3 epsilon&CD3 gamma |
| Host | Mouse |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Molecular Mass | The protein has a predicted MW of 15.34 kDa (CD3E) and 15.73 kDa (CD3G). Due to glycosylation, the protein migrates to 18-23 kDa and 25-27 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Asp22-Asp108(CD3E) & Gln23-Ser116(CD3G) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 0.1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P22646(CD3E)&P11942(CD3G) |
| Amino Acid Sequence | Recombinant Mouse CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed as a heterodimer of CD3E and CD3G by co-expression in HEK293. Subunit CD3E is fused with a Flag tag at the C-terminus and subunit CD3G is fused with a His tag at the C-terminus. It contains Asp22-Asp108 (CD3E) and Gln23-Ser116 (CD3G). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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