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Biotinylated Human CD73/NT5E Protein (CD7-HM473B)

SKU: BTL-KB-P-00779 | Host: Human | Brand: Kactus Bio
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Product Description

Cat Number CD7-HM473B
Category Recombinant Protein
Pack Size 100UG
Description Recombinant Biotinylated Human CD73/NT5E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp27-Lys547.
Target CD73/NT5E
Host Human
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Scientific Background CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Molecular Mass The protein has a predicted MW of 60.92 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Sl Sequence Trp27-Lys547
Solubility Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Appearance Lyophilized
Endotoxin Levels Less than 1 EU per μg by the LAL method.
Expression System HEK293
Gene Id P21589-1
Amino Acid Sequence Recombinant Biotinylated Human CD73/NT5E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp27-Lys547.
Storage -20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Pdf View Document
Note The product is for research use only
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