Product Description
| Cat Number | DKK-HM101 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr32-His266. |
| Target | DKK1 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE |
| Scientific Background | Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. |
| Molecular Mass | The protein has a predicted MW of 26.87 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Thr32-His266 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | O94907 |
| Amino Acid Sequence | Recombinant Human DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr32-His266. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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