Product Description
| Cat Number | E-BC-K804-M_96T |
|---|---|
| Category | Cell Metabolism Assay Kit |
| Pack Size | 96T |
| Detection Method | Colorimetric method |
| Research Field | Oxidative stress;Metabolic Diseases;Glycolysis and Lipid Metabolism |
| Sensitivity | 0.02 μmol/L |
| Detection Range | 0.02-5.0 μmol/L |
| Sample Type | animal tissue;cell |
| Assay Length | 70 min |
| Target Synonyms | NAD+/NADH |
| Reader | Microplate reader |
| Test Principle | Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results. |
| Storage | This product can be stored at 2~8°C/-20°C~-80°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Technical Manual | View Document |
| Msds | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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