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Mouse INS-Ab (Insulin Autoantibody) CLIA Kit

SKU: BTL-EL-AK-00467 | Brand: Elabscience
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Product Description

Cat Number E-CL-M0436_48T
Category Chemiluminescence Assay Kit
Pack Size 48T
Detection Method Chemiluminescence
Sensitivity 18.75 pg/mL
Detection Range 31.25-2000 pg/mL
Reactivity Mouse
Sample Type Serum, plasma and other biological fluids
Assay Length 3 h 30 min
Target Synonyms Anti-Insulin Antibody;Antiinsulin;Insulin Antibodies;
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IAA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IAA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IAA. You can calculate the concentration of Mouse IAA in the samples by comparing the RLU value of the samples to the standard curve.
Storage 2-8℃/-20℃
Standard Curve View Document
Technical Manual View Document
Note The product is for research use only
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