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Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit

SKU: BTL-EL-AK-00084 | Brand: Elabscience
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Product Description

Cat Number E-CL-R0589_24T
Category Chemiluminescence Assay Kit
Pack Size 24T
Detection Method Chemiluminescence
Research Field Cancer, Epigenetics and Nuclear Signaling, Developmental biology, Stem cells
Sensitivity 37.5 pg/mL
Detection Range 62.5-4000 pg/mL
Reactivity Rat
Sample Type Serum, plasma and other biological fluids
Assay Length 3 h 30 min
Uniprot Q9Z2J9
Target Synonyms AML3;CBFA1;CCD;CCD1;OSF2;PEA2aA;PEBP2A1;PEBP2A2;PEBP2aA;PEBP2aA1;
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RUNX2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat RUNX2. You can calculate the concentration of Rat RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.
Storage 2-8℃/-20℃
Standard Curve View Document
Technical Manual View Document
Note The product is for research use only
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