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Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit

SKU: BTL-EL-AK-00023 | Brand: Elabscience
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Product Description

Cat Number E-CL-R0720_48T
Category Chemiluminescence Assay Kit
Pack Size 48T
Detection Method Chemiluminescence
Research Field Cancer, Cardiovascular, Metabolism, Neuroscience
Sensitivity 7.5 pg/mL
Detection Range 12.5-800 pg/mL
Reactivity Rat
Sample Type serum, plasma and other biological fluids
Assay Length 3 h 30 min
Uniprot P23363
Target Synonyms ANON2;BULN2;
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the RLU value of the samples to the standard curve.
Storage 2-8℃/-20℃
Standard Curve View Document
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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