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Rat GFAP (Glial Fibrillary Acidic Protein) CLIA Kit

SKU: BTL-EL-AK-00012 | Brand: Elabscience
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Product Description

Cat Number E-CL-R0740_24T
Category Chemiluminescence Assay Kit
Pack Size 24T
Detection Method Chemiluminescence
Research Field Cancer, Neuroscience, Signal transduction, Stem cells, Tags and Cell Markers
Sensitivity 37.5 pg/mL
Detection Range 62.5-4000 pg/mL
Reactivity Rat
Sample Type serum, plasma and other biological fluids
Assay Length 3 h 30 min
Uniprot P47819
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GFAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GFAP. You can calculate the concentration of Rat GFAP in the samples by comparing the RLU value of the samples to the standard curve.
Storage 2-8℃/-20℃
Standard Curve View Document
Technical Manual View Document
Msds View Document
Product Url View Document
Note The product is for research use only
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