Human EGFRVIII Protein, Ultra Low Endotoxin (EG8-HM254-UL)
SKU: BTL-KB-P-01279 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | EG8-HM254-UL |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human EGFRVIII Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu25-Ser378. |
| Target | EGFRVIII |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
| Molecular Mass | The protein has a predicted MW of 64.58 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
| Sl Sequence | Leu25-Ser378 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 0.01 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | NP_001333870.1 |
| Amino Acid Sequence | Recombinant Human EGFRVIII Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu25-Ser378. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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