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Human FcgRII(Receptor II For The Fc Region Of Immunoglobulin G) ELISA Kit

SKU: BTL-ELK-E-02922 | Brand: ELK
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Product Description

Cat Number ELK2071
Category ELISA Kit
Pack Size 1EA
Elisa Type Sandwich
Sensitivity 16.58 pg/mL
Standard 3000 pg/mL
Detection Range 46.88-3000 pg/mL
Sample Type serum, plasma and other biological fluids
Assay Length 3.5h
Research Area CD & Adhesion molecule;Infection immunity;
Target Synonyms CD32; FCGR2A; FCG2; FCGR2A1; FCGR2; FCGR2B; FCGR2C; Fc Fragment Of IgG, Low Affinity IIa, Receptor; Low affinity immunoglobulin gamma Fc region receptor II-a
Test Principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgRII in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve View Document
Technical Manual View Document
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Note The product is for research use only
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