Product Description
| Cat Number | ELK2663 |
|---|---|
| Category | ELISA Kit |
| Pack Size | 1EA |
| Elisa Type | Sandwich |
| Sensitivity | 0.066 ng/mL |
| Standard | 10 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology; |
| Uniprot | Q9JHR7 |
| Target Synonyms | Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IDE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IDE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IDE in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Standard Curve | View Document |
| Technical Manual | View Document |
| Product Url | View Document |
| Note | The product is for research use only |
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