Product Description
| Cat Number | EPH-HM1A3 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human EPHA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Gln541. |
| Target | EPHA3 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Erythropoietin‑producing hepatocellular carcinoma cell surface type‑A receptor 3 (EPHA3) has been found to promote the proliferation and survival of prostate cancer (PCa) cell lines and prostate tumor development in nude mice. The interaction of AR and SP1 contributes to regulate EPHA3 expression, and the SP1 binding sites (‑295~‑261) in the EPHA3 core promoter region is crucial to the regulation of EPHA3 expression in response to androgen hormone stimuli. |
| Molecular Mass | The protein has a predicted MW of 59.9 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Glu21-Gln541 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P29320-1 |
| Amino Acid Sequence | Recombinant Human EPHA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Gln541. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
Share Item: