Non-biotinylated Human GARP&Latent TGF Beta 2 Complex Protein (GAT-HM402)
SKU: BTL-KB-P-01589 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | GAT-HM402 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human GARP&Latent TGF Beta 2 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of GARP.It contains His20-Leu628(GARP) and Leu21-Ser414(Latent TGF Beta 2). |
| Target | GARP&Latent TGF Beta 2 Complex |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |
| Molecular Mass | The protein has a predicted MW of 70.3 kDa (GARP) and 45.53 kDa (Latent TGF Beta 2). Due to enzyme lysis and glycosylation, the protein migrates to 72-77 kDa (GARP), 13 kDa and 46-48 kDa (Latent TGF Beta 2) based on Bis-Tris PAGE result. |
| Sl Sequence | His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 0.1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q14392(GARP)&P61812-1(Latent TGF Beta 2) |
| Amino Acid Sequence | Recombinant Human GARP&Latent TGF Beta 2 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of GARP.It contains His20-Leu628(GARP) and Leu21-Ser414(Latent TGF Beta 2). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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